Journal: Journal of Cellular and Molecular Medicine

Article Title: Trpv4 induces collateral vessel growth during regeneration of the arterial circulation

doi: 10.1111/j.1582-4934.2008.00579.x

Figure Lengend Snippet: Increased expression of Trpv4 after 4αPDD treatment of cultured porcine endothelial cells. (A) Transcriptional levels of Trpv4 were not increased significantly after treatment of porcine endothelial cells (PAECs) with 37 μM 4αPDD. (B) Cultures of PAECs were double‐labelled with Phalloidin (red) and antibody against Trpv4 (green). 4αPDD treatment stimulates Trpv4 expression in a dose‐dependent manner. Fluorescence intensity given in arbitrary units (AU)/μm2 was quantified from representative images (n= 6) using ImageJ software (http://rsb.info.nih.gov/ij/). Nuclei were counterstained with Draq5 (blue). Scale bars: 40 μm. (C) Western blot analysis of cultured PAECs treated with 4αPDD showed increased expression of Trpv4 protein. Trpv4 expression was compared to solvent controls (EtOH) and protein extracts from kidney (pig), and normalized to Pan‐Actin. Relative protein levels were quantified using QuantityOne software (BioRad) (n= 5). (Values are given in mean ± S.E.M.; *P < 0.05)

Article Snippet: Relative amount of proteins was normalized to Pan‐Actin (1:1000; Cell‐Signalling, #4968, Danvers, MA, USA) and was quantified using QuantityOne software (v4.6.3, BioRad, Munich, Germany).

Techniques: Expressing, Cell Culture, Fluorescence, Software, Western Blot, Solvent